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Glycerol stimulation of chlorophyll synthesis, embryogenesis, and carboxylation and sucrose metabolism enzymes in nucellar callus of Hamlin' sweet orange

Identifieur interne : 003894 ( Main/Exploration ); précédent : 003893; suivant : 003895

Glycerol stimulation of chlorophyll synthesis, embryogenesis, and carboxylation and sucrose metabolism enzymes in nucellar callus of Hamlin' sweet orange

Auteurs : J. C. V. Vu [États-Unis] ; R. P. Niedz ; G. Yelenosky

Source :

RBID : Pascal:93-0342719

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English descriptors

Abstract

Phosphoenolpyruvate carboxylase (PEPCase) and sucrose phosphate synthase (SPS) were active in nucellar calli of «Hamlin» sweet orange, Citrus sinensis (L.) Osbeck, grown on media containing either 5% sucrose or 2% glycerol as the primary carbon source. Activities of the enzymes, however, were much higher in the glycerol-grown tissues than those grown in sucrose. Glycerol, in addition, stimulated embryogenesis and chlorophyll biosynthesis and initiated the onset of ribulose bisphosphate carboxylase-oxygenase (Rubisco) activity. These stimulatory effects, which were not observed in sucrose-grown calli, became more obvious when calli grown on glycerol-containing, agar-solidified medium were transferred and grown in liquid suspension medium containing 2% glycerol


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Le document en format XML

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<s1>ARS, horticultural res. lab.</s1>
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<country>États-Unis</country>
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<name sortKey="Niedz, R P" sort="Niedz, R P" uniqKey="Niedz R" first="R. P." last="Niedz">R. P. Niedz</name>
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<term>Biosynthesis</term>
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<term>Citrus sinensis</term>
<term>Embryonic development</term>
<term>Enzymatic activity</term>
<term>Glycerol</term>
<term>Metabolism</term>
<term>Phosphoenolpyruvate carboxylase</term>
<term>Ribulosebisphosphate carboxylase</term>
<term>Sucrose synthase</term>
<term>Sucrose-phosphate synthase</term>
<term>Tissue culture</term>
<term>Vegetals</term>
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<term>Métabolisme</term>
<term>Culture tissu</term>
<term>Végétal</term>
<term>Développement embryonnaire</term>
<term>Chlorophylle</term>
<term>Biosynthèse</term>
<term>Sucrose-phosphate synthase</term>
<term>Phosphoenolpyruvate carboxylase</term>
<term>Ribulosebisphosphate carboxylase</term>
<term>Activité enzymatique</term>
<term>Glycérol</term>
<term>Citrus sinensis</term>
<term>Agrume</term>
<term>Sucrose synthase</term>
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<div type="abstract" xml:lang="en">Phosphoenolpyruvate carboxylase (PEPCase) and sucrose phosphate synthase (SPS) were active in nucellar calli of «Hamlin» sweet orange, Citrus sinensis (L.) Osbeck, grown on media containing either 5% sucrose or 2% glycerol as the primary carbon source. Activities of the enzymes, however, were much higher in the glycerol-grown tissues than those grown in sucrose. Glycerol, in addition, stimulated embryogenesis and chlorophyll biosynthesis and initiated the onset of ribulose bisphosphate carboxylase-oxygenase (Rubisco) activity. These stimulatory effects, which were not observed in sucrose-grown calli, became more obvious when calli grown on glycerol-containing, agar-solidified medium were transferred and grown in liquid suspension medium containing 2% glycerol</div>
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